OUR PAPERS

Arranged thematically below - or find us on PubMed or GoogleScholar.
(meta)effectors  |   CRISPR-Cas and phage   | other

(META)EFFECTORS

Mount HO, Urbanus ML, Zangari F, Gingras A-C, Ensminger AW. The Legionella pneumophila effector PieF modulates mRNA stability through association with eukaryotic CCR4-NOT. mSphere. 2024 Dec 19:e0089124. doi: 10.1128/msphere.00891-24. Online ahead of print. PMID: 39699231.

Patel DT, Stogios PJ, Jaroszewski L, Urbanus ML, Sedova M, Semper C, Le C, Takkouche A, Ichii K, Innabi J, Patel DH, Ensminger AW, Godzik A, Savchenko A. Global atlas of predicted functional domains in Legionella pneumophila Dot/Icm translocated effectors. Mol Syst Biol. 2025 Jan;21(1):59-89. doi: 10.1038/s44320-024-00076-z. Epub 2024 Nov 19. PMID: 39562741.

Mount HO, Urbanus ML, Sheykhkarimli D, Coté AG, Laval F, Coppin G, Kishore N, Li R, Spirohn-Fitzgerald K, Petersen MO, Knapp JJ, Kim D-K, Twizere J-C, Calderwood MA, Vidal M, Roth FP, Ensminger AW. A comprehensive two-hybrid analysis to explore the Legionella pneumophila effector-effector interactome. mSystems. 2024 Dec 17;9(12):e0100424. doi: 10.1128/msystems.01004-24. Epub 2024 Nov 11. PMID: 39526800.

Hershkovitz D, Chen EJ, Ensminger AW, Dugan AS, Conway KT, Joyce AC, Segal G, Isberg RR. Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway. mSphere. 2024 Sep 25;9(9):e0022224. doi: 10.1128/msphere.00222-24. Epub 2024 Aug 21 PMID: 39166849.

Urbanus ML, Zheng TM, Khusnutdinova AN, Banh D, O'Connor Mount H, Gupta A, Stogios PJ, Savchenko A, Isberg RR, Yakunin AF, Ensminger AW. A random mutagenesis screen enriched for missense mutations in bacterial effector proteins. G3 Genes|Genomes|Genetics. 2024 Sep 4;14(9):jkae158. doi: 10.1093/g3journal/jkae158. PMID: 39028840.

Kaneko T, Stogios PJ, Ruan X, Voss C, Evdokimova E, Skarina T, Chung A, Liu X, Li L, Savchenko A, Ensminger AW, Li SS. Identification and characterization of a large family of superbinding bacterial SH2 domains. Nat Commun. 2018 Oct 31;9(1):4549. doi: 10.1038/s41467-018-06943-2. PubMed PMID: 30382091.

Valleau D, Quaile AT, Cui H, Xu X, Evdokimova E, Chang C, Cuff ME, Urbanus ML, Houliston S, Arrowsmith CH, Ensminger AW, Savchenko A. Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella pneumophila. Cell Rep. 2018 Apr 10;23(2):568-583. doi: 10.1016/j.celrep.2018.03.060. PubMed PMID: 29642013.

Urbanus ML, Quaile AT, Stogios PJ, Morar M, Rao C, Di Leo R, Evdokimova E, Lam M, Oatway C, Cuff ME, Osipiuk J, Michalska K, Nocek BP, Taipale M, Savchenko A, Ensminger AW. Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila. Mol Syst Biol. 2016 Dec 16;12(12):893. doi: 10.15252/msb.20167381. PubMed PMID: 27986836.

Ensminger AW. Legionella pneumophila, armed to the hilt: justifying the largest arsenal of effectors in the bacterial world. Curr Opin Microbiol. 2016 Feb;29:74-80. doi: 10.1016/j.mib.2015.11.002. Epub 2015 Dec 19. Review. PubMed PMID: 26709975.

Quaile AT, Urbanus ML, Stogios PJ, Nocek B, Skarina T, Ensminger AW, Savchenko A. Molecular Characterization of LubX: Functional Divergence of the U-Box Fold by Legionella pneumophila. Structure. 2015 Aug 4;23(8):1459-1469. doi: 10.1016/j.str.2015.05.020. Epub 2015 Jul 2. PubMed PMID: 26146184.

Quaile AT, Stogios PJ, Egorova O, Evdokimova E, Valleau D, Nocek B, Kompella PS, Peisajovich S, Yakunin AF, Ensminger AW, Savchenko A. The Legionella pneumophila effector Ceg4 is a phosphotyrosine phosphatase that attenuates activation of eukaryotic MAPK pathways. J Biol Chem. 2018 Mar 2;293(9):3307-3320. doi: 10.1074/jbc.M117.812727. Epub 2018 Jan 4. PubMed PMID: 29301934.

Morar M, Evdokimova E, Chang C, Ensminger AW, Savchenko A. Crystal structure of the Legionella pneumophila Lem10 effector reveals a new member of the HD protein superfamily. Proteins. 2015 Dec;83(12):2319-25. doi: 10.1002/prot.24933.  Epub 2015 Oct 14. PubMed PMID: 26426142.

Ensminger AW, Isberg RR. E3 ubiquitin ligase activity and targeting of BAT3 by multiple Legionella pneumophila translocated substrates. Infect Immun. 2010 Sep;78(9):3905-19. doi: 10.1128/IAI.00344-10. Epub 2010 Jun 14. PubMed PMID: 20547746.

Ensminger AW, Isberg RR. Legionella pneumophila Dot/Icm translocated substrates: a sum of parts. Curr Opin Microbiol. 2009 Feb;12(1):67-73. doi: 10.1016/j.mib.2008.12.004. Epub 2009 Jan 20. Review. PubMed PMID: 19157961.

CRISPR-CAS

Nicholson B, Deecker SR, Ensminger AW. Mobile element integration reveals a chromosome dimer resolution system in Legionellales. mBio. 2022 Dec 20;13(6):e0217122. doi: 10.1128/mbio.02171-22. Epub 2022 Oct 31. PubMed PMID: 36314797.

Deecker SR, Urbanus ML, Nicholson B, Ensminger AW. Legionella pneumophila CRISPR-Cas suggests recurrent encounters with one or more phages in the family Microviridae. Appl Environ Microbiol. 2021 Aug 11;87(17):e0046721. doi: 10.1128/AEM.00467-21. PubMed PMID: 34132590.

Deecker SR, Ensminger AW. Type I-F CRISPR-Cas Distribution and Array Dynamics in Legionella pneumophila. G3 Genes|Genomes|Genetics. 2020 Mar 5;10(3):1039-1050. doi: 10.1534/g3.119.400813. PubMed PMID: 31937548.

Rao C, Chin D, Ensminger AW. Priming in a permissive type I-C CRISPR-Cas system reveals distinct dynamics of spacer acquisition and loss. RNA. 2017 Oct;23(10):1525-1538. doi: 10.1261/rna.062083.117. Epub 2017 Jul 19. PubMed PMID: 28724535.

Rao C, Guyard C, Pelaz C, Wasserscheid J, Bondy-Denomy J, Dewar K, Ensminger AW. Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to the pathogen. Cell Microbiol. 2016 Oct;18(10):1319-38. doi: 10.1111/cmi.12586. Epub 2016 Mar 31. PubMed PMID: 26936325.

other

Lin JD, Stogios PJ, Abe KT, Wang A, MacPherson J, Skarina T, Gingras A-C, Savchenko A, Ensminger AW. Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila. mBio. 2023 Oct 31;14(5):e0151023. doi: 10.1128/mbio.01510-23. PMID: 37819088.

Saha S, Ojobor CD, Li ASC, Mackinnon E, North OI, Bondy-Denomy J, Lam JS, Ensminger AW, Maxwell KL, Davidson AR. F-Type Pyocins Are Diverse Noncontractile Phage Tail-Like Weapons for Killing Pseudomonas aeruginosa. J Bacteriol. 2023 Jun 27;205(6):e0002923. doi: 10.1128/jb.00029-23. Epub 2023 Jun 1. PMID: 37260386

Chin D, Deecker SR, Ensminger AW, Heinrichs DE. Draft Genome Sequence of Staphylococcus chromogenes ATCC 43764, a Coagulase-Negative Staphylococcus Strain with Antibacterial Potential. Microbiol Resour Announc. 2021 Jun 3;10(22):e0049221. doi: 10.1128/MRA.00492-21. Epub 2021 Jun 3. PMID: 34080903.

Chin D, Goncheva MI, Flannagan RS, Deecker SR, Guariglia-Oropeza V, Ensminger AW, Heinrichs DE. Coagulase-negative staphylococci release a purine analog that inhibits Staphylococcus aureus virulence. Nat Commun. 2021 Mar 25;12(1):1887. doi: 10.1038/s41467-021-22175-3. PMID: 33767207.

Rothenbroker M, McConnell EM, Gu J, Urbanus ML, Samani SE, Ensminger AW, Filipe CDM, Li Y. Selection and Characterization of an RNA-Cleaving DNAzyme Activated by Legionella pneumophila. Angew Chem Int Ed Engl. 2021 Feb 23;60(9):4782-4788. doi: 10.1002/anie.202012444. Epub 2021 Jan 15. PMID: 33188548.

Dey R, Mount H, Ensminger AW, Tyrrell GJ, Ward LP, Ashbolt NJ. Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada. Emerg Infect Dis. 2019 Nov;25(11):2104-2107. doi: 10.3201/eid2511.190522. PMID: 31625866.

Boamah DK, Zhou G, Ensminger AW, O'Connor TJ. From Many Hosts, One Accidental Pathogen: The Diverse Protozoan Hosts of Legionella. Front Cell Infect Microbiol. 2017 Nov 30;7:477. doi: 10.3389/fcimb.2017.00477. PMID; 29250488.

Knox NC, Weedmark KA, Conly J, Ensminger AW, Hosein FS, Drews SJ; Legionella Outbreak Investigative Team. Unusual Legionnaires' outbreak in cool, dry Western Canada: an investigation using genomic epidemiology. Epidemiol Infect. 2017 Jan;145(2):254-265. doi: 10.1017/S0950268816001965. PMID: 27760576.

Ali SS, Soo J, Rao C, Leung AS, Ngai DH, Ensminger AW, Navarre WW. Silencing by H-NS potentiated the evolution of Salmonella. PLoS Pathog. 2014 Nov 6;10(11):e1004500. doi: 10.1371/journal.ppat.1004500. PMID; 25375226.

Ensminger AW. Experimental evolution of pathogenesis: "patient" research. PLoS Pathog. 2013;9(5):e1003340. doi: 10.1371/journal.ppat.1003340. PMID: 23737744.

Khan MA, Knox N, Prashar A, Alexander D, Abdel-Nour M, Duncan C, Tang P, Amatullah H, Dos Santos CC, Tijet N, Low DE, Pourcel C, Van Domselaar G, Terebiznik M, Ensminger AW, Guyard C. Comparative Genomics Reveal That Host-Innate Immune Responses Influence the Clinical Prevalence of Legionella pneumophila Serogroups. PLoS One. 2013 Jun 27;8(6):e67298. doi: 10.1371/journal.pone.0067298. PMID: 23826259.

Rao C, Benhabib H, Ensminger AW. Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics. PLoS One. 2013 May 22;8(5):e64129. doi: 10.1371/journal.pone.0064129. PMID: 23717549.

Ensminger AW, Yassin Y, Miron A, Isberg RR. Experimental evolution of Legionella pneumophila in mouse macrophages leads to strains with altered determinants of environmental survival. PLoS Pathog. 2012;8(5):e1002731. doi: 10.1371/journal.ppat.1002731. PMID: 22693450.